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The most common cell cultures used in antibody production today are mammalian cells as they produce glycoproteins with established therapeutic efficiency. The cloning of antibody-encoded genes into different expression hosts solved the problem of frequently lost antibody encoding genes during long-term cultivation and allowed for large-scale therapeutic production optimization. Over the years, the production of monoclonal antibodies has shifted from a reliance on hybridoma cell lines to recombinant technologies. Antibody production – eligible cell cultures Moreover, compared to other mammalian cells used in bioprocessing, CHO cells are able to produce a very high level of recombinant protein. In comparison, CHO cells have shown to be very stable in antibody expression with almost no contamination risks. This includes human cell lines which run at a greater risk for viral contamination and subsequent transmission in monoclonal antibody mAb production. To use CHO cells to express recombinant proteins in bioprocessing was a huge step forward compared to achievements with any other animal cell. Due to their large success, manufacturers use CHO cells in the different steps of recombinant antibody production: Transfection, recombinant protein production, purification and antibody expression. The use of CHO cells for the production of recombinant antibodies has led to a boost in the production process of recombinant protein therapeutics. Through CHO cell antibody production, it became possible to derive therapeutic antibodies as treatments for diseases like cancer, multiple sclerosis, asthma, HIV or neuroblastoma. CHO Cell Antibody Production with evitria CHO cell antibody production The American Psychological Association lists "committed suicide" as a term to avoid because it "frame suicide as a crime". In 2011, in an article calling for changing the language used around suicide entitled “Suicide and language: Why we shouldn't use the ‘C’ word,” the Centre for Suicide Prevention in Canada found that the normal verb in scholarly research and journalism for the act of suicide was commit and argued for destigmatizing terminology related to suicide. Suicide in which the reason is that the person feels that they are not part of society is known as egoistic suicide. A special case of this is extended suicide, where the murder is motivated by seeing the murdered persons as an extension of their self. In a murder–suicide (or homicide–suicide), the individual aims at taking the lives of others at the same time. It may or may not involve exact planning or intent. Suicidal ideation is thoughts of ending one's life but not taking any active efforts to do so. This is in contrast to euthanasia, where another person takes a more active role in bringing about a person's death. Assisted suicide occurs when one individual helps another bring about their own death indirectly via providing either advice or the means to the end. Attempted suicide or non-fatal suicidal behavior amounts to self-injury with at least some desire to end one's life that does not result in death. Suicide, derived from Latin suicidium, is "the act of taking one's own life". Suicide is often seen as a major catastrophe for families, relatives, and other nearby supporters, and it is viewed negatively almost everywhere around the world. In the 20th and 21st centuries, suicide has been used on rare occasions as a form of protest, and kamikaze and suicide bombings have been used as a military or terrorist tactic. It remains a criminal offense in some countries. Suicide and attempted suicide, while previously illegal, are no longer so in most Western countries. Sati, a practice outlawed by the British in India, expected a Hindu widow to immolate herself on her husband's funeral pyre, either willingly or under pressure from her family and society. During the samurai era in Japan, a form of suicide known as seppuku ( 腹切り, harakiri) was respected as a means of making up for failure or as a form of protest. The Abrahamic religions traditionally consider suicide as an offense towards God due to belief in the sanctity of life. Views on suicide have been influenced by broad existential themes such as religion, honor, and the meaning of life. In the Western world, attempts are more common among young people and women. Non-fatal suicide attempts may lead to injury and long-term disabilities. There are an estimated 10 to 20 million non-fatal attempted suicides every year. Europe had the highest rates of suicide by region in 2015. Suicide is generally most common among those over the age of 70 however, in certain countries, those aged between 15 and 30 are at the highest risk. Rates of suicide are generally higher among men than women, ranging from 1.5 times higher in the developing world to 3.5 times higher in the developed world. In a given year, this is roughly 12 per 100,000 people. Īpproximately 1.5% of all deaths worldwide are by suicide. This makes suicide the 10th leading cause of death worldwide. Suicides resulted in 828,000 deaths globally in 2015, an increase from 712,000 deaths in 1990. Common methods of suicide include hanging, pesticide poisoning, and firearms. The most commonly adopted method of suicide varies from country to country and is partly related to the availability of effective means. Although crisis hotlines are common resources, their effectiveness has not been well studied. Effective suicide prevention efforts include limiting access to methods of suicide such as firearms, drugs, and poisons treating mental disorders and substance abuse careful media reporting about suicide and improving economic conditions. Those who have previously attempted suicide are at a higher risk for future attempts. Some suicides are impulsive acts due to stress (such as from financial or academic difficulties), relationship problems (such as breakups or divorces), or harassment and bullying. Mental disorders (including depression, bipolar disorder, schizophrenia, personality disorders, anxiety disorders), physical disorders (such as chronic fatigue syndrome), and substance abuse (including alcoholism and the use of and withdrawal from benzodiazepines) are risk factors. Suicide is the act of intentionally causing one's own death. Read moreĬontractual Obligations and Commitments Our. Read moreįactors Affecting Our Performance We. Read moreįurthermore, we plan to continue. Read moreĬash flows from operating activities. Read moreĪdditional limitations of ARR include. Interest Expense Interest expense increased. Read moreĪdjusted Gross Margin We believe. provides a foundation for businesses, developers, IT professionals and individuals to store, back up and archive data, host content, manage media. Cash flow from financial activities can be defined as the net amount of cash transactions used in funding activities. With over two billion gigabytes of data storage under management, the company currently works with more than 500,000 customers in over 175. Read moreĪs customers generate, store, and. Backblaze cash flow from financial activities from 2020 to 2023. The Backblaze Storage Cloud provides a foundation for businesses, developers, IT professionals, and individuals to build applications, host content, manage media, back up and archive data, and more. Read moreĪccordingly, we recorded a gain. Read moreĬapitalized Internal-Use Software, Net We. Read moreĬonsumption-based revenue is variable and. Read moreĬash used in investing activities. Variable consideration, which contains estimates. Read moreįor additional details, see the. Read moreĬomputer Backup (subscription-based arrangements) revenue. Personnel-related costs associated with customer. Read moreīecause of these limitations, when. Read moreįor our subscription arrangements, we. Backblaze, Inc., a storage cloud platform, provides businesses and consumers cloud services to store, use, and protect data in the United States and. Key Business Metrics We monitor the key business metrics set forth below to help us evaluate our business and growth trends, establish budgets, measure the effectiveness of our sales and marketing efforts, and assess operational efficiencies. The following table presents a reconciliation of gross profit, the most directly comparable financial measure stated in accordance with GAAP, to adjusted gross profit, for each of the periods presented: Adjusted EBITDA Our management uses adjusted EBITDA to assess our operating performance. Non-GAAP Financial Measures To supplement our financial statements, which are prepared and presented in accordance with generally accepted accounting principles in the United States, or GAAP, we provide investors with non-GAAP financial measures including adjusted gross margin and adjusted EBITDA, each as defined below. Our future capital requirements will depend on many factors, including our total revenue growth rate, the timing and the amount of cash received from customers, the expansion of sales and marketing activities, the timing and extent of spending to support development efforts, the price at which we are able to purchase or lease infrastructure equipment, the introduction of platform enhancements, and the continuing market adoption of our platform.Ĭash used in operations increased during the year ended December 31, 2022, as compared to the same period in 2021 primarily due to increased spending in support of our expanded research and development and sales and marketing spending to support business growth. Other Inside Backblaze, Inc.'s 10-K Annual Report: Start by heating a medium to large pot with about 3-5 inches of water depending on the size of the pot. EGGS BENEDICT: I urge you to watch this video before beginning to poach the eggs.Make it with smoked salmon, crab cakes, or Canadian bacon, but whatever you do, make it!Īlso, how great would these be to surprise your hunny with on Sunday morning? There are also tips on how you can poach the eggs ahead of time if you’re cooking for a large crowd.Īnd that’s it! Simple blender hollandaise sauce to use on your eggs benedict this weekend. Here is a video that I found really useful and here is another one that i’ve used in the past. Which is basically letting the water come to a boil and then stirring it and adding the egg to the center. It’s so much easier to poach them when they’re fresher because they stay together in the water. My tips to making better poached eggs? Reach all the way in the back of the egg section at your grocery store. Okay, it took me a WHILE to get these right so I don’t want you to get super frustrated if you can’t get them right the first time. I like and usually have salted butter on hand so I didn’t need to season my sauce much, you can use unsalted butter and just add in a ½ teaspoon of salt and it will work out just the same. Melted butter, egg yolks, a tablespoon of lemon juice, and a teeny tiny pinch of cayenne is all you need to make my hollandaise. If you’re wondering about the ingredients there are just a few. Perfectly smooth hollandaise sauce every. Give it a whiz and then stream in the slightly cooled melted butter while the blender runs. Then it’s just as simple as adding in the yolks, lemon juice and cayenne. This allows the blender to stay warm while you’re adding in the ingredients. Nothing special, just water that I heated up in my water kettle. Before starting the recipe, I fill the blender up with about 4 cups of boiling water and just let it sit for 1-2 minutes while the butter melts in a saucepan on the stove. And after making it both over the double boiler and in a blender I realized the blender hollandaise sauce was far more delicious and FOOLPROOF than the stove top version.īut I do have a secret to making perfect hollandaise sauce in the blender. I have been tweaking this recipe for some time now. Let’s talk about that homemade hollandaise sauce. Holy Mackerel, i’m forever in love with them. But my favorite? Anees surprised me with a small birthday brunch get together at a really nice steak house this past December and you guys, they make crab cake eggs benedict. Whats your favorite way to eat eggs benedict? I love and most usually get the smoked salmon. There’s no way my Sunday could get more perfect than that.Īnd at this point are you really shocked to hear that both mastering poached eggs and making blender hollandaise sauce were on my culinary bucket list? This culinary bucket list is working out to be such a great thing, I mean poached eggs with runny egg yolk and quick hollandaise for Sunday brunch all the time? YES. Buttery, toasted english muffins with smoked salmon topped with a nicely poached egg (which up until recently wasn’t done very nicely), and drizzled with my homemade blender hollandaise sauce. Sure we love our spinach and mushroom stuffed omelets with homemade hash browns, which has become a Sunday ritual, and we always enjoy our veggie-loaded breakfast casserole, and the occasional stack of pancakes.īut nothing beats the classic eggs benedict for a special Sunday brunch. When it comes to weekend breakfast, Anees and I love a little variety. We’re talking real hollandaise sauce, creamy, smooth, and rich – made right in the blender. Gone are the days of making hollandaise sauce in a double boiler and whisking until your arm fell off. We’re talking about just 4 simple ingredients and about 5 minutes to make from start to finish. And to top it all off, it’s a cinch to whip up. I know what you’re thinking, hollandaise sauce, in the blender? YES, my friends. I am so excited to be bringing you one of my all time favorite breakfast foods - eggs benedict topped with homemade blender hollandaise sauce. This sauce requires just 4 simple ingredients and takes about 5 minutes to make! Homemade hollandaise is perfect to use on eggs benedict for Sunday brunch! A simple blender hollandaise sauce recipe. It’s an excellent choice for concealed carry, and it is equally in its element at the range. If you’re looking for a concealable pistol, but don’t want to sacrifice capacity, reach for an XD 3” Sub-Compact. 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This shows all of the patches you have in your queue. hg qseries demonstrates this: $ hg qseries -v -sĠ A bug-123456-fix: Bug 123456: A brief summary of the changes you have made.ġ A bug-341896-fix: Bug 341896: The fix, summarized briefly. MQ allows you to keep your unrelated changes isolated from each other. If you look at the new patch file you have exported, you'll notice that it only contains the changes for your bug-341896-fix mq entry. $ hg export qtip > ~/bug-341896-fix.patch $ hg qrefresh -m "Bug 341896: The fix, summarized briefly." The process is exactly the same: $ hg qnew bug-341896-fix While the patch is being reviewed, you might want to work on another bug. $ hg export qtip > ~/bug-123456-fix.patch $ hg qrefresh -m "Bug 123456: A brief summary of the changes you have made." The following commands demonstrate how to create an MQ entry for a single bug fix, record the changes you make, and turn them into a diff file named "bug-123456-fix.patch" that is ready to be attached to the corresponding Bugzilla bug. Remove an unapplied patch from your queue. Update the current patch to include your latest uncommitted changes. Make a new empty patch and give it a name. This allows changing binary files in your patches. To enable MQ, put this in your Mecurial.ini file for Windows (see mozilla-build in Windows Build Prerequisites) or $HOME/.hgrc file: ĭon't forget the git line. hg/patches directory under the repository. You can apply them as Mercurial changesets, unapply them, edit them, and when they're done, turn them into permanent changesets and push them.Įach repository has its own queue of patches managed by MQ. The MQ extension lets you treat a stack of patches as works-in-progress. The output of a developer (on a good day, anyway) is patches. The mqext extension can make this much easier.
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